Abstract Background The parasitic mite Varroa destructor is considered the major pest of the European honey bee (Apis mellifera) and responsible for declines in honey bee populations worldwide. Exploiting the full potential of gene sequences becoming available for V. destructor requires adaptation of modern molecular biology approaches to this non-model organism. https://www.lightemupsequences.com/limited-save-Custom-Congratulations-Banner-p4839-hot-choice/